![]() If FlowJo™ can’t find the FCS files it may be because the file has changed location, or the metadata has been altered.įCS Error in FlowJo™ v10 due to broken pathįlowJo™ does not save your FCS files as part of the Workspace. If your data is already organized into folders, FlowJo™ will automatically create and organize these into groups in your workspace. **Location of Add Samples tool in FlowJo™**** You can add samples with the Add Samples button (two blue test tubes with an orange plus) located both in the FlowJo™ ribbon and in the upper left corner to the right of the undo/redo buttons. Simply drag and drop your file onto the FlowJo™ v10 workspace (Where it says “drag samples here” is a good place to start!) Headers are readable by humans, but the rest of the data is storage in compressed binaries that represent all the complex information contained in the columns and rows, like: fluorescence intensity values, forward scatter, side scatter, and more.Įach file has a unique “finger print” based on the data it contains. The meta data of this file contains four major required components: HEADER, TEXT, DATA, and ANALYSIS (Seamer). Each set includes data from a single sample or acquisition run. The file may contain one or more data sets. The FCS file provides a detailed description of: We are committed to creating instrument agnostic software now and in the future.įCS stands for “Flow Cytometry Standard.” The goal of the standard is to provide a uniform file format that allows files created by one type of acquisition hardware and software to be read by any other (Spidlen). Our own director of Research & Development, Josef Spidlen PhD, is an author of the most recent ISAC data standards and wrote the leading paper on creating uniform flow cytometry standard file types that can be created and read universally. Yes! FlowJo™ is completely instrument-agnostic and accepts FCS and LMD (embedded FCS format) files from any cytometer.
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![]() Unfortunately two of his most solid anchors disappear during the course of this retrospective work, and there are eulogies of remembrance, of moments shared – yet more acknowledgement and appreciation – that are beautiful to behold.Īs I say, KING-CAT is a very kind comic, very brave and very intimate. I used to like to explore so often I don’t make the time anymore. John also does a lot of walking to stave off or alleviate those symptoms, by day and at night, popping down alleys purely because he’s never done so before. So those early days in SF were open and free, and the creative spirit of the city inspired me.” New surroundings, while fear-inducing at first, often-times relieved my symptoms – everything was fresh and hadn’t yet taken on a multi-layered patina of anxiety. Moving home is a double-edged sword for someone with John’s OCD as he explains succinctly in the back of his move early on here to San Francisco: Vilification met with genuine care and compassion. “Brother,” asks the Hippie Girl, innocently, “What happened to your smile?!” The one-page prologue, ‘Hippie Girl’, is highly unusual except in its retrospective self-recrimination at his anger after being ravaged by OCD (it was drawn last year, but occurred during this period circa 2006) emphasised by the love heart between “Hippie” and “Girl” and its direct, cut-through-the-bullshit, priority punchline: Indeed, his very occasional allusions to his mental health within the body of KING-CAT itself cause him nothing but more grief and guilt. You’re going to witness remarkably little of that in his comics – which is as extraordinarily restrained as the comics are controlled – but it’s ever so real as the notes in the back and the whole of his HOSPITAL SUITE make abundantly clear. Other times they’re brand-new discoveries, and it is quite the journey, both spiritually and geographically as John uproots himself, his wife and his cat to move house such vast distances that they take five full days of self-driving.Īnd that’s where the anchor comes in, because John needs anchors like Maisie and Misun and his Dad so desperately, and that’s where the love comes back too: giving this love and appreciation is John’s way of staying sane, of holding on hard to hope when the crushing adversity is so crippling at times that he cannot create. ”That warm light inside that tells you it’s safe Sometimes they’re pivotal moments, like his history with drinking (it wasn’t good he stopped) sometimes they’re reflections that have since taken on new meaning to him along his journey. ![]() They’re almost always dated, both the memories and their commitment to paper. John is as likely to recall memories from many moons ago as he is to tell you more recent tales. Porcellino also lists his ‘King-Cat Top 40’s, scattered with more tiny hearts, as a positive way of acknowledging and publicly appreciating anyone and anything that has brought him joy in the making of each semi-annual KING-CAT comic or during their intervening months: friends, music, pictures, books, places, sensations, more light, more nature, more moments, and memories too. Sometimes he’ll simply tell you about a tree. Breezes carry scents and he notes those too.įoxes, skunks and squirrels are observed, sometimes sought after, and flora is cherished as much as fauna. Sometimes he evokes them verbally, poetically often he leaves his clean and precise pictures, already full of space, to do that instead. The weather, as well: sunshine, wind, rain and snow. Light comes constantly under his appreciative gaze, during the day and at night and those hours of spectacle in between. Throughout these pure, direct and above all honest, mostly autobiographical short stories, John receives love from his wife Misun and his cat Maisie in a quiet, unfussy and far from cloying fashion, and he returns this adoration to Misun, Maisie and – with awe and appreciation – to the abundant wonder which he perceives all around him. I think it’s perfect, both for Diogenes and for John. Porcellino gives Diogenes a couple of tattoos: an anchor on his right arm, a love heart on his left. “Diogenes said:”I’m searching for the bones of your father, but I can’t tell them apart from those of a slave.”” “Alexander the Great came along and asked him what he was doing… “One day Diogenes was sorting through a pile of bones… Yet it’s not without its moments of comedy, especially where insight’s involved. It’s also very brave and astonishingly intimate. It is clear, concise and enormously thoughtful. From Lone Mountain (£16-99, Drawn & Quarterly) by John Porcellino. Featuring John Porcellino, Jason, Nicolas Wild, Brendan Fletcher, Karl Kerschl, Jordie Bellaire, Vanesa Del Rey and even Eddie Campbell, after a fashion. ![]() Assist Trophy Ī horde of Excitebike riders appear as a collective Assist Trophy in Brawl. Official artwork of an Excitebike in Brawl. ![]() Ultimately, that position went to the Ice Climbers, and in the released game, Excitebike is only an obtainable trophy. It was briefly rumoured that, along with Balloon Fighter, 'Excitebiker' was going to be a playable character in Super Smash Bros. This game is no longer able to be purchased after the shut down of the Wii Shop Channel on January 31, 2019. While being mostly similar to the NES entry, the game has an isometric perspective and features original tracks, as well as a now defunct online component. The final entry in the series was the download-only WiiWare game Excitebike: World Rally. This was followed up by Excitebots: Trick Racing, which focused on animal themed robotic vehicles and even more over-the-top gameplay and presentation. The game is a departure from the series by the introduction of cars and trucks, as well as a less realistic a more arcadey feel to the game. The first was Excite Truck, released in 2006 for Wii. The series then changed hands to Monster Games, who primarily developed all original releases going forward. The game utilized the N64 rumble pak to simulate the bumps and enviroments on the road while playing. The game is less of a sequel and more of a 3D remake of the original game, complete with recreations of previous tracks with the new axis. The series laid dormant for 16 years until a new game was released in 2000 for Nintendo 64, titled Excitebike 64. The first time for the e-Reader accessory in 2002, and again in 2004 on an actual cartridge as part of the Classic NES Series. The game was also rereleased for Game Boy Advance twice. Excitebike, a reskin with Mario characters exclusively for the Satelleview add-on to the Super Famicom titled Excitebike: Bun Bun Mario Battle Stadium, and a remake for Nintendo 3DS with new camera controls titled 3D Classics: Excitebike. Notable examples include an arcade port titled Vs. This game in particular has been ported and remade numerous times over the decades. If the player runs into other races or lands at the wrong angle after a jump, they crash and have to slowly get back on the bike before resuming the race. If the bike overheats, it slows to a crawl until it cools down. Alongside shifting lanes, jumping off ramps and avoiding other racers, the player has to constantly monitor their engine temperature. The game is an early example of a "simulation" game, with attempts at realistic physics and controls. The goal of the game is making it to the finish line before the opponents. Shigeru Miyamoto and Toshihiko Nakago, the co-directors of the game, traveled from their homes to the development offices in Tokyo and stayed their for several days, often sharing a hotel room because of the high volume of tourists at the time. The first game titled Excitebike debuted in 1984 on the Famicom in Japan, then in other regions in 1985 on the Nintendo Entertainment System. 6 Games with elements appearing in the Super Smash Bros. ![]() They are the most fundamental part of the project and are the smallest part of the project. It consists of levels to further breakdown activities into smaller units. The WBS is a hierarchical structure of all the works to be done in the project. After that, a person from the OBS is assigned to the project along with a calendar. The project is added under the respective division in the EPS. ![]() The project is the collection of all activities and information linked to it that constitutes a plan for the completion of the construction project. This includes the company hierarchy and the people responsible for the projects in the EPS. The basic structure of a project created on Primavera P6 is as follows: Enterprise Project StructureĪt first, the EPS is created, which means the structure of the company that is executing the project. It allows all project team members to be able to track live-schedule and provide progress information and feedback on a daily basis to improve the schedule. Team MemberĪs mentioned before, Team Member is a built-in tool on top of Primavera P6. In addition, Primavera P6 can generate multiple float paths to project end, allowing the project management to Longest Path, 2nd Longest Path, and 3rd Longest Path, and other driving factors of project milestones. As a result, controlling the project can become quite easy by identifying the critical path. The software can identify critical activities and automatically form a critical path of the project. In conclusion, budget variations can be easily identified throughout the project with the help of Primavera P6. Cost PlanningĮvery activity and resource has a cost associated with it. When you allocate resources to activities in Primavera P6, it shows a resource histogram, with the help of which you can level resources and avoid uneven distribution of resources. As a result, increasing or optimizing these resources can reduce delays and reduce activity duration. Primavera P6 offers the user with multiple uses such as: Time ManagementĪctivities in a construction project can be planned based on the human and non-human resources available. In conclusion, all the project team members can see and track live schedule activities on a daily basis and provide feedback to the power user and project scheduler for improvement. Furthermore, the Team Member tool of Primavera P6 is a built-in tool that allows the site team to easily report work progress daily with the use of a computer, a phone, or an iPad. The user of Oracle Primavera P6 management tool can easily identify and analyze the performance status of driving activities, milestones, longest path, 2nd longest path, other float paths, earned value indices, and more with the use of advanced reporting features of Primavera P6. Additionally, a feature unique to this software is that multiple baselines can be set and maintained on the program. On-site progress is compared with the baseline project. Once the initial project plan is created in Primavera P6, it can then be set and maintained as a project baseline and then progressed & monitored regularly. Furthermore, the program allows the user to load resources into the activities, which allows the user to get a clearer image of the resource allocation. This is vital information for fast track projects to start activities in parallel and avoid delays. This program helps in finding the actual relationship and sequence between activities to be executed on-site. Planning and SchedulingĪn ideal project plan or baseline is always constructed at the start of the project, for which Primavera P6 can be used. However, a planning engineer mainly uses primavera P6 for the Planning, Monitoring, and Controlling project schedule and resources. Each step is crucial for project success and to achieve the goal of the project scope. Areas of applicationĪ project’s lifecycle consists of 5 steps: Initiation, Planning, Execution, Monitoring and Control, and Closing. It is used for handling multiple projects and can store up to 100,000 activities with countless resources and target plans. Primavera P6 is a software owned by Oracle and used by Project Management Professionals. With that in mind, one of these sophisticated and essential tools is Primavera P6. ![]() The construction industry around the world is moving towards more advanced tools and techniques to handle the ever-growing demands, tackle the increase in workload, and efficiently manage resources. Just like an orange or banana peel you have created a coherent texture. ![]() Unfold the cube and note where each edges letter is, this is how most textures are laded out. If done right all toy should have a total of twelve letters, each paired up. A good example of showing this is to make a second cube and label each edge with a matching letter. Typically in the 3D world you will produce a 3D shape then make cuts along the edges and vertices to flatten the image in a layout that makes sense. What you just did was apply a "Texture" or "skin" to the cube, though not very intuitively. Notice that the drawings warp around the cube, at some parts the drawings look out of place once they reach an edge. Once you draw a few designs fold the cube up and look at each of the faces. Before you assemble the cube draw some designs on the paper while it's flatten. The area that makes up the space inside the square is called a "Face" or also known as "polygon". Looking at your cut-out you will see lines that make up six squares in a cross shape pattern (some designs very), each line is called an "Edge", the intersection of each line or point is called "Vertices". Print and cut out the design, once done you are ready to learn how things work in 3D space. Follow the instructions here: Paper Cube. The best way to understand 3D space and modeling is making a cube from a flat piece of paper. ![]() I hope that this document would help people do more modeling and texture work for the future and maybe have a way to crowd source new submissions and fixes (looking at you clipping on Charr). Chapter three is where some example textures are given and how to mod the textures to get the desired look. Chapter two is the process of getting started, such as downloads and programs. Chapter one is concepts of 3D modeling, such as textures, normal maps, models and image displays. I have broken down the process into three chapters for ease of use and understanding. This might make the game nearly unplayable while the program is running. Because of the nature of the program that is used to mod the textures you might experience an extreme loss of frames per second. Programs and actions done here might be in direct violation of Arenanet's terms of service and end-user license agreement, so use at your own risk.ģ. I am simply posting what steps "I" did to get things working for my system, and the risks that "I" had to take for my system.Ģ. The documentation and methods presented here-forth is to not a license or liberty to hold me accountable for any damages or problems that might incur while following the document and procedure. I would like to start off with some disclaimers before I go any further in modding the Guild Wars 2 game.ġ. "There are so many factors involved that it is never wise to attempt to judge intrinsic value to the last eighth or even point." Phil Fisher "Valuation of a business is - it’s not reducible to any formula where you can actually put in the variables perfectly." Warren Buffett “ No number, on its own, can capture valuation.” Mark Urquart "There's no such thing as precise intrinsic value." Mohnish Pabrai “It is better to be approximately right, than precisely wrong.” Warren Buffett "Do not try for a pin-pointed mathematical approach that creates an illusory certainty out of an unknowable future." T Rowe Price The decision should be obvious." Charlie Munger "We never sit down, run the numbers out and discount them back to net present value. "It's always difficult to rule out something beyond the reach of your equations." Brian Greene “We’d argue there’s a lot of false precision in our business and that the best investments don’t require a financial model that goes out five significant digits.” Ira Rothberg "Business value is a soft number: in our own case, two equally well-informed observers might make judgments more than 10% apart." Warren Buffett You just don’t want to imagine that within a tenth of a percent or a nickel a share or even a dollar a share that you really can have that much precision." Chris Davis So what we tend to come up with are what we call ranges of fair value. You have an enormous amount of uncertainty that you have to price in. ![]() There’s just amazing false precision in that. "When we look and value a business, we don’t come out with an estimate that, you know, company X is worth $34 a share. “Any attempt to value businesses with precision will yield values that are precisely inaccurate.” Seth Klarman We just see something that obviously is better than anything else around that we understand - and then we act." Warren Buffett "If you need to use a computer or calculator to make the calculation, you shouldn't buy it.It should scream at you.we do not sit down with spreadsheets and do all that sort of thing. “The reason our ideas haven’t spread faster is they’re too simple.” Charlie Munger ![]() ![]() the various factors involved may be so conflicting that the conclusion finally drawn is no better than a snap judgement would have been." Garfield Drew, 1941 " Simplicity or singleness of approach is a greatly underestimated factor of market success. As soon as the attempt is made to watch a multiplicity of factors even though each one has some element to justify it, one is only too likely to become lost in a maze of contradictory implications. Whenever calculus is brought in, or higher algebra, you could take it as a warning signal that the operator was trying to substitute theory for experience." Ben Graham "In 44 years of Wall Street experience and study I have never seen dependable calculations made about common stock values, or related investment policies, that went beyond simple arithmetic or the most elementary algebra. ![]() The actual calculation process is done by the JavaScript file we have included. The tag is used to create the button to calculate the BMI. The placeholder attribute defines the text to be displayed in the input fields when no user input is present. But also the letter “e” (due to Mathematical reasons - exponential) and period “.” are allowed. The “type” attributes limit the user input to those input fields to only numbers. The “id” attributes of these input tags are used to identify the two inputs given by the user separately. We are using two tags to take those two inputs from the user. To calculate the BMI, we need two inputs from the user, Since we are getting the fonts from the internet on the run, they won’t be visible if you are viewing the web page offline. The last two tags are used to include the fonts used for our web page. Parsing means reading the HTML source (index.html) and this reading is done by the browser, line by line. The attribute “defer” is a boolean attribute says the relevant script to execute only when the page has finished parsing. The “src” attribute specifies the target JavaScript script file. The tag is used to included external scripts required for the functioning of this web page. In the case of main.css, that file is having a relationship of being an external stylesheet to this HTML document, hence the “stylesheet” value for the “rel” attribute. The “rel” means the relationship of that external file to this HTML document. The tag is used to link external files required for the displaying of this web page. The tag defines what would be the title displayed on the browser tab. The encoding is variable-length and uses 8-bit code units.” By definition, “UTF-8 (U from Universal Character Set + Transformation Format - 8-bit) is a character encoding capable of encoding all possible characters (called code points) in Unicode. The charset attribute sets the character encoding used in this document (HTML page). The tag contains important metadata (data about data) and some other important information regarding to the web page. Although this is not essential to put, it is highly recommended since there are a lot of advantages of putting it. ![]() The second line states the language in which this web page is written. ![]() The first line helps browsers understand that this. ![]() But the album also shed its predecessor's nod to harder electronics for a joyride in pop-dance bliss. Sure, Discovery is littered with anthems for the club, where no little ones would be allowed. I've always liked to believe that Daft Punk's most groundbreaking album is a nod to the simple joy of being a kid. Its nasty, dumb, gorgeous, and so 2016 it hurts.- Ezra Marcus Tapping into a vein of bratty bravado, he careens past toothless PG-13 peacocking into the kind of unrepentant sleaze that made him successful in the first place, culminating in a breathtaking stretch on the bridge where he sneers about fucking your girl, guzzling cocaine, and buying his mom a station wagon all in the same breath. The steel-tipped drums bristle against the warm synths and keys, while the song's sexy, restrained groove sends The Weeknd into savage mode overdrive. "Starboy" succeeds by cultivating tension between gloss and grit. Instead, they strip the beat down to elegiac keys, incandescent synths, and a razor-wire drum loop-their simplest, sharpest production work since contributing militant percussion to Yeezus. Gone is the tepid live instrumentation that bogged down Daft Punk's collaborations with Pharrell. Many saw the collaboration as a naked cash grab, but "Starboy" is a leap beyond both sides' previous forays into 80s dance-funk. "Starboy" is a frontal assault on Daft Punk's storied dance music lineage-a snotty millennial banger better suited to Snapchat stories than Essential Mixes. The backlash against Daft Punk's 2016 collaboration with The Weeknd, encapsulated in the comments on this Resident Advisor news piece from September, is hardly surprising. "I have no idea who is actually under those helmets, but the real Daft Punk died years ago." Blame Daft Punk for that one, at least in part.- Max Mertensġ3. West took some heat from Parkinson's advocacy groups for that line, but "On Sight" feels like a heat-seeking missile headed straight to the critics who thought he was incapable of still provoking with his music. They don't relent until the 1:16 mark, when the soulful chorus from the Holy Name of Mary Choral Family's rendition of "He'll Give Us What We Really Need" provides a brief reprieve from its confrontational pace. ![]() The track's snarling, buzz-saw synths that grab the listener's attention from the get-go. Half an hour late, he opened with the frenzied one-two punch of " Black Skinhead " and " New Slaves "-both of which he had performed on Saturday Night Live a few weeks earlier-before later playing another Daft Punk-assisted cut, "On Sight," which also enlisted the services of Mike Dean and Benji B. Though the Governors Ball Music Festival had been rained out two days earlier, turning the field into a muddy quagmire for the rest of the weekend, the anticipation in the crowd was electrifying. On June 3, 2016, I was at Randall's Island Park in New York City when Kanye West debuted a quarter of the songs that would make up his sixth studio album, Yeezus. More than anything, it's a reminder that the golden robots will always be there, spinning our favorite memories back to us one more time.- Ezra Marcusġ8. All told, the catalogue includes 102 tracks comprising an artistic vision as distinct as the duo's silhouettes on that iconic Coachella billboard. Some are all-time classics some, not so much. ![]() ![]() We included all five studio albums (including the Tron soundtrack), as well as numerous remixes, B-sides, and songs they've produced for artists like Kanye West-though if there were multiple versions of the same track, we only chose the best one. Which is to say, here at THUMP, we've decided to rank every Daft Punk song because arbitrary lists are the only things that make sense anymore. So as the world crumbles around us, we build fortresses in our mind. In an era where chaos reigns, Daft Punk provide stability. People play their hits at weddings, bar mitzvahs, and graduation parties because they want to remember those moments forever, and the songs feel timeless enough to last that long. The same themes occur over and over Thomas Bangalter and Guy-Manuel de Homem-Christo are drawn like moths to the flames of romance, youth, and parties-emotional and physical settings where time melts away. Daft Punk carve out the fragmentary hearts of old songs, preserving their essence in digital amber. It costs 89.95 for a single shared USB port, 159.95 for 2 USB ports, 399.95 for up to 10 shared USB ports, and 699.95 for an unlimited number of shared USB ports. Software license can be purchased based on the number of shared USB ports. To share the dongle, you should start USB Network Gate and open the Local Devices tab. USB Network Gate is a paid software which includes a free trial of 14 days. Uses a complex algorithm to aggregate NICs and configure speed and duplex settings. The hostname you are connecting to, raspberrypi. ![]() Transmits network packets using the Hash algorithm, which selects the same NIC slave for each destination MAC address. Transmits network packets sequentially to each network interface card in order to distribute the internet traffic among all the NICs. Similar to Balance-tlb, but offers additional load balancing for incoming IPv4 traffic. Outgoing traffic is distributed based on the current load for each interface relative to the interface's maximum speed. If the interface fails, a slave interface takes over the MAC address of the failed interface. Incoming traffic is received by the current interface. Transmits the same network packets to all the network interface cards. If this primary interface becomes unavailable, the secondary interface will become active. Secured login and multi-factor authenticationĪll traffic is sent and received using the interface that was first added to the trunking group.Support Milestone Surveillance Platform.NAS Smart Surveillance System Solutions.NDR Solutions against Targeted Ransomware.Software-defined Platform Total Solution.Support Platform9’s Managed OpenStack Solution.Veeam-Ready and Virtualization Certifications.With Linux and ZFS, QuTS hero supports advanced data reduction technologies for further driving down costs and increasing reliablility of SSD (all-flash) storage. QuTS hero is the operating system for high-end and enterprise QNAP NAS models. WIth Linux and ext4, QTS enables reliable storage for everyone with versatile value-added features and apps, such as snapshots, Plex media servers, and easy access of your personal cloud. QTS is the operating system for entry- and mid-level QNAP NAS. ![]() This will ideally allow travelers to get from Caloocan to España in just five minutes, according to the Department of Transportation. Table showing various Minute measurements converted to seconds. (SPOT.ph) The first section of the North Luzon Expressway Connector finally opened to the public on March 27, right in time for the Holy Week. According the to SI definition, the second is the duration of 9,192,631,770. The table below contains conversion factors to convert any time value into seconds using multiplication. ![]() For example, 1 second can be written as 1 s or 1 sec. The second has historically been defined as 1/60 of a minute or 1/86,400 of a day. There are 0.01666667 minutes in a second. Seconds can be abbreviated as s, and are also sometimes abbreviated as sec. The second is the SI base unit for time in the metric system. The second has historically been defined as 1/60 of a minute or 1/86,400 of a day.Īccording the to SI definition, the second is the duration of 9,192,631,770 periods of the radiation corresponding to the transition between the two hyperfine levels of the unperturbed ground state of the caesium 133 atom. |